Procedure for Biochemical Oxygen Demand (BOD) Test
This is an important test because it reveals the amount of dissolved oxygen organisms need to break down organic matter in a water sample over a certain time period.
Materials Needed:
Black light blocking B.O.D. bottle with seal cap.
To conduct this test in the field you need to...
1. Find an area of water deep enough to put the entire bottle in.
2. Fill the bottle up with water, slowly turning it upside down in the water so that all the oxygen leaves the bottle and there is only water within.
3. Remove the bottle from the water at the same time putting the seal cap to prevent any oxygen from getting in and messing up the results.
Upon returning to the class:
1. You must let the sample sit for an incubation period of 5 days
2. After 5 days, take the seal cap off of the sample and pour the water into a beaker.
3. Once the water is in the beaker, take the probe, connect one end to the sensor and place the other end in the beaker.
4. Let the sensor fully calculate and then record the results
Pictures:
B.O.D. Bottle
Matt and Jared with all the materials to conduct the test:
Significance: This test is significant because it tells us the amount dissolved oxygen required by an organism to dissolve organic material. It is an effective test for determining organic pollution.
Typical readings:
Typical B.O.D. values for a clean and unpolluted body of water, such as Hoit Pond Marsh, are usually anywhere under 1 mg/L
This is an important test because it reveals the amount of dissolved oxygen organisms need to break down organic matter in a water sample over a certain time period.
Materials Needed:
Black light blocking B.O.D. bottle with seal cap.
To conduct this test in the field you need to...
1. Find an area of water deep enough to put the entire bottle in.
2. Fill the bottle up with water, slowly turning it upside down in the water so that all the oxygen leaves the bottle and there is only water within.
3. Remove the bottle from the water at the same time putting the seal cap to prevent any oxygen from getting in and messing up the results.
Upon returning to the class:
1. You must let the sample sit for an incubation period of 5 days
2. After 5 days, take the seal cap off of the sample and pour the water into a beaker.
3. Once the water is in the beaker, take the probe, connect one end to the sensor and place the other end in the beaker.
4. Let the sensor fully calculate and then record the results
Pictures:
B.O.D. Bottle
Matt and Jared with all the materials to conduct the test:
Significance: This test is significant because it tells us the amount dissolved oxygen required by an organism to dissolve organic material. It is an effective test for determining organic pollution.
Typical readings:
Typical B.O.D. values for a clean and unpolluted body of water, such as Hoit Pond Marsh, are usually anywhere under 1 mg/L
Jared Roth and Matt Campbell - 12/5/12